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first strand cdna synthesis kit  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology first strand cdna synthesis kit
    First Strand Cdna Synthesis Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abscript+ii+cdna+first-strand+synthesis+kit+rk20400/ABscript+II+cDNA+First+Strand+Synthesis+kit/pm42789758-75-12-16
    Average 98 stars, based on 218 article reviews
    first strand cdna synthesis kit - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: DNA hypermethylation of abscisic-acid-related genes helps enhance the cold tolerance of tetraploid rice.
    Article Snippet: rights reserved.. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.

    Article Title: Small-Molecule-Mediated Depletion of the Dual-Specificity Tyrosine Phosphorylation-Regulated Kinase 1B (DYRK1B) Protein Scaffold
    Article Snippet: .. Afterward, cDNA was synthesized, using the ABScript II cDNA First-Strand Synthesis Kit with Oligo d(T)23VN primers (ABclonal, Düsseldorf, Germany). .. Quantitative PCR was conducted using the 2× Universal SYBR Green FASt qPCR Mix (ABclonal, Düsseldorf, Germany), Mic Tubes and Caps (bio molecular systems, Australia) and the Q-qPCR instrument (Quantabio, Beverly, MA).

    Real-time Polymerase Chain Reaction:

    Article Title: DNA hypermethylation of abscisic-acid-related genes helps enhance the cold tolerance of tetraploid rice.
    Article Snippet: rights reserved.. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.

    SYBR Green Assay:

    Article Title: DNA hypermethylation of abscisic-acid-related genes helps enhance the cold tolerance of tetraploid rice.
    Article Snippet: rights reserved.. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.

    Reverse Transcription:

    Article Title: The novel role of MEG3 in Glaesserella parasuis-infected porcine alveolar macrophages: Modulating apoptosis via MDM2-mediated ubiquitination and degradation of ILF3 to suppress CFLAR transcription.
    Article Snippet: Glaesserella parasuis (G. parasuis) is an important opportunistic pathogen that poses a threat to the swine industry, and the induction of host cell apoptosis is one of the key pathogenic mechanisms by which G. parasuis invades and damages the host.. Long non-coding RNAs (lncRNAs) exert diverse and crucial biological functions in cells.. Maternally expressed gene 3 (MEG3), a lncRNA widely involved in cell proliferation, apoptosis, and immune responses, has been demonstrated to be associated with G. parasuis infection.

    Article Title: BBX maintains mammalian brain development by repressing p53-mediated p21 expression.
    Article Snippet: To construct expression vectors for BBX, p53, and p21, total RNA was isolated from primary NSCs using RNAiso Plus reagent (Takara, Japan). .. The isolated RNA (2 μg) and oligo(dT) primers (Bionics, South Korea) were used to synthesize first-strand cDNA using a reverse transcription kit (ABclonal, USA) according to the manufacturer’s instructions. ..

    Article Title: Comparative effects of semaglutide tirzepatide and retatrutide on renal fibrosis in UUO and aged mice
    Article Snippet: .. Total RNA was isolated from kidney tissues and HK-2 cells with a commercial kit (19211ES60, Yeasen, China) and reverse-transcribed into cDNA (RK20400, ABclonal, China). .. Subsequent qPCR amplification (11203ES08, Yeasen, China) was carried out with an initial denaturation step (95°C for 3min), followed by 40 cycles of 95°C for 5s and 60°C for 30s.

    Isolation:

    Article Title: Targeting TLR4/NF-κB/NLRP3 signaling with 6-hydroxyflavanone mitigates amyloid-β-induced Alzheimer's disease in mice.
    Article Snippet: The total RNA from the hippocampal sample was isolated using TRI reagent (Cat # 9108; DSS Takara, Dalian, China), and the purity and concentration of the RNA samples were estimated using a Nanodrop reader. .. Further, cDNA was synthesised from the isolated RNA using a cDNA synthesis kit (Cat # RK20400; ABclonal, USA). .. The target gene amplification was performed by qRT-PCR using the QuantStudio 5 (Applied Biosystems, USA) and SYBR Green (Cat # RK21204; ABclonal, USA).

    Article Title: BBX maintains mammalian brain development by repressing p53-mediated p21 expression.
    Article Snippet: To construct expression vectors for BBX, p53, and p21, total RNA was isolated from primary NSCs using RNAiso Plus reagent (Takara, Japan). .. The isolated RNA (2 μg) and oligo(dT) primers (Bionics, South Korea) were used to synthesize first-strand cDNA using a reverse transcription kit (ABclonal, USA) according to the manufacturer’s instructions. ..

    Article Title: Comparative effects of semaglutide tirzepatide and retatrutide on renal fibrosis in UUO and aged mice
    Article Snippet: .. Total RNA was isolated from kidney tissues and HK-2 cells with a commercial kit (19211ES60, Yeasen, China) and reverse-transcribed into cDNA (RK20400, ABclonal, China). .. Subsequent qPCR amplification (11203ES08, Yeasen, China) was carried out with an initial denaturation step (95°C for 3min), followed by 40 cycles of 95°C for 5s and 60°C for 30s.

    cDNA Synthesis:

    Article Title: Targeting TLR4/NF-κB/NLRP3 signaling with 6-hydroxyflavanone mitigates amyloid-β-induced Alzheimer's disease in mice.
    Article Snippet: The total RNA from the hippocampal sample was isolated using TRI reagent (Cat # 9108; DSS Takara, Dalian, China), and the purity and concentration of the RNA samples were estimated using a Nanodrop reader. .. Further, cDNA was synthesised from the isolated RNA using a cDNA synthesis kit (Cat # RK20400; ABclonal, USA). .. The target gene amplification was performed by qRT-PCR using the QuantStudio 5 (Applied Biosystems, USA) and SYBR Green (Cat # RK21204; ABclonal, USA).

    Article Title: Comparative pathogenesis, tissue tropism, and immune dynamics of infectious bronchitis virus genotypes GI‑1 and GI‑24 in broiler chickens.
    Article Snippet: .. Quantitative analysis of cytokine responses following IBV infection Total RNA extracted from various tissue samples (Respiratory tissues and kidney) collected at different days post infection (dpi) was used for cDNA synthesis with the ABScript II cDNA First Strand Synthesis Kit (ABclonal TechnologyTM, USA) following the manufacturer’s instructions. .. After the measurement of the concentration of the synthesized cDNA using Nanodrop one (Thermo Scientific, USA), it was diluted at a ratio of 1:10 using nuclease-free water.

    Article Title: Alda-1 Alleviates the Cognitive Deficits in D-galactose-induced Aging Mice by Regulating Oxidative Stress, Neuroinflammation, and Mitochondrial Biogenesis.
    Article Snippet: Although mitochondrial aldehyde dehydrogenase 2 (ALDH2) is involved in aging and age-related neurodegenerative diseases, the impact of ALDH2 on age-related cognitive deficits and its underlying mechanisms have not been fully elucidated.. This study aimed to explore the effects of ALDH2 on cognitive deficits in D-galactose-induced aging mice and illustrate the potential molecular mechanisms.. We treated D-galactose-induced aging mice with Alda-1 (a potent agonist of ALDH2) for eight weeks and observed the alterations in behavior, levels of oxidative stress and neuroinflammation, mitochondrial function, and expression of mitochondrial biogenesis-related genes.

    Analysis:

    Article Title: Comparative pathogenesis, tissue tropism, and immune dynamics of infectious bronchitis virus genotypes GI‑1 and GI‑24 in broiler chickens.
    Article Snippet: .. Quantitative analysis of cytokine responses following IBV infection Total RNA extracted from various tissue samples (Respiratory tissues and kidney) collected at different days post infection (dpi) was used for cDNA synthesis with the ABScript II cDNA First Strand Synthesis Kit (ABclonal TechnologyTM, USA) following the manufacturer’s instructions. .. After the measurement of the concentration of the synthesized cDNA using Nanodrop one (Thermo Scientific, USA), it was diluted at a ratio of 1:10 using nuclease-free water.

    Infection:

    Article Title: Comparative pathogenesis, tissue tropism, and immune dynamics of infectious bronchitis virus genotypes GI‑1 and GI‑24 in broiler chickens.
    Article Snippet: .. Quantitative analysis of cytokine responses following IBV infection Total RNA extracted from various tissue samples (Respiratory tissues and kidney) collected at different days post infection (dpi) was used for cDNA synthesis with the ABScript II cDNA First Strand Synthesis Kit (ABclonal TechnologyTM, USA) following the manufacturer’s instructions. .. After the measurement of the concentration of the synthesized cDNA using Nanodrop one (Thermo Scientific, USA), it was diluted at a ratio of 1:10 using nuclease-free water.

    Tissue:

    Article Title: Comparative pathogenesis, tissue tropism, and immune dynamics of infectious bronchitis virus genotypes GI‑1 and GI‑24 in broiler chickens.
    Article Snippet: .. Quantitative analysis of cytokine responses following IBV infection Total RNA extracted from various tissue samples (Respiratory tissues and kidney) collected at different days post infection (dpi) was used for cDNA synthesis with the ABScript II cDNA First Strand Synthesis Kit (ABclonal TechnologyTM, USA) following the manufacturer’s instructions. .. After the measurement of the concentration of the synthesized cDNA using Nanodrop one (Thermo Scientific, USA), it was diluted at a ratio of 1:10 using nuclease-free water.



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    Image Search Results


    Journal: iScience

    Article Title: Comparative effects of semaglutide tirzepatide and retatrutide on renal fibrosis in UUO and aged mice

    doi: 10.1016/j.isci.2026.117174

    Figure Lengend Snippet:

    Article Snippet: Total RNA was isolated from kidney tissues and HK-2 cells with a commercial kit (19211ES60, Yeasen, China) and reverse-transcribed into cDNA (RK20400, ABclonal, China).

    Techniques: Recombinant, Staining, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, RNA Sequencing, Sequencing, Software